[Occupational injury incidence due to biological material in a tertiary referral public hospital in Santa Fe province]. / Incidencia de accidentes laborales como potencial riesgo biológico en un hospital publico de tercer nivel de atención de la provincia de Santa Fe.

UNLABELLED: Occupational accidents due to biological materials cause significant harm to workers and institutions. OBJECTIVE: To estimate the risk of accidents in doctors and nurses at the Hospital Provincial del Centenario (HPC) and particularly in Emergency Medical Service (EMS) between 2009 and 2013. METHODS: retrospective cohort study from the survey information of injuries by accidents with biohazard in doctors and nurses workers from HPC. RESULTS: medical workers reported 45.7% (95% CI 37.2 to 54.3%) of accidents and nurses 38.4% (from 30.3 to 47.1%). The incidence rate ratio (IRR) (doctors / nurses) (2009-2013) = 0.953 (0.661-1.373). EMS workers ages and seniority were significantly lower than those of other care areas taken together. Reports of accidents from the EMS were 34.1% (95% CI: 26.2 to 42.6%) of the total. EMS workers had age and seniority average significantly less than workers in other services. In EMS, the IRR (doctors / nurses) (2009-2013) = 6.337 (2.264 -17.733). SGM medical workers ages and seniority were significantly lower than those of nurses working in this service. CONCLUSION: The EMS was particularly vulnerable to occupational injuries, medical workers have a higher risk of accidents compared to nursing workers. The younger age and lower seniority of medical workers could be related at least partly to these findings.

Factores de riesgo sexual y parenteral en los encuestados durante la campaña contra hepatitis virales 2013, Rosario, Argentina / Sexual and parenteral risk factors in subjects surveyed during a campaign against viral hepatitis (2013), Rosario, Argentina

La enfermedad hepática viral constituye un problema y un desafío para los médicos y sus pacientes. En agosto de 2013 en la ciudad de Rosario se efectuó la Campaña de Detección y Vacunación de Hepatitis Virales. Este trabajo tiene como objetivo caracterizar los principales factores de riesgo sexual (FRS) y parenteral (FRP) de los voluntarios asistentes a dicha campaña. Se llevó a cabo un estudio de corte transversal utilizando como instrumento un cuestionario validado. Se encontró que el 37,1%(IC 95%: 32,8-41,6%) de los encuestados refería al menos un FRS, siendo el más prevalente la presencia de relaciones sexuales inseguras con el 68,9% (61,6-75,6%). El 35,7% (31,4-40,1%) presentó al menos un FRP, destacándose aquí la utilización de piercings y tatuajes con el 74,6% (67,4-80,9%) y el 48% (40,3-55,7%) respectivamente. En los individuos mayores de 60 años,los hombres hicieron referencia a mayor exposición a FRS que las mujeres (37,2% vs 16,2%; p= 0,019); mientras que para los menores de 40 años las mujeres presentaron mayor exposición a FRP (64,5% vs 45,0%; p= 0,016). La prevalencia de factores de riesgo aumentó al disminuir la edad de los respondedores. Los jóvenes entre 18 y 30 años mostraron además, asociación entre los FRS y FRP (p= 0,01). Los factores de riesgo estudiados resultan diferenciarse según grupos etarios y género. Es necesario contemplar una educación dirigida a distintos grupos poblacionales, focalizando en las debilidades propias de cada uno

Viral liver disease is a problem and a challenge for physicians and their patients. A campaign for the detection and vaccination againstviral hepatitis was performed in August 2013 in the city of Rosario, Argentina. This work aims to characterize the main sexual (SRF)and parenteral (PRF) risk factors among those volunteers attending the campaign. A cross-sectional study was conducted using avalidated questionnaire. It was found that 37.1% (95% CI: 32.8-41.6%) of the respondents mentioned at least one SRF; the mostprevalent factor was the presence of unsafe sex with 68.9% (61.6-75.6%). On the other hand, 35.7% (31.4-40.1%) referred atleast one PRF, of which the most frequent were the use of piercings and tattoos with 74.6% (67.4-80.9%) and 48% (40,3-55.7%)respectively. Men reported higher exposure to SRF than women (37.2% vs 16.2%, p= 0.019) among individuals older than 60 years;while for those under 40 years, women were more likely to show PRF (64.5% vs 45.0%, p= 0.016). The prevalence of risk factorswas increased while decreasing age of respondents. Young people between 18 and 30 also showed association between SRF and PRF(p = 0,01). Risk factors studied varied according to age groups and genders. It is necessary to provide education targeted to differentpopulation groups, focusing on their particular characteristics and weaknesses of each of them

Factores de riesgo sexual y parenteral en los encuestados durante la campaña contra hepatitis virales 2013, Rosario, Argentina / Sexual and parenteral risk factors in subjects surveyed during a campaign against viral hepatitis (2013), Rosario, Argentina

La enfermedad hepática viral constituye un problema y un desafío para los médicos y sus pacientes. En agosto de 2013 en la ciudad de Rosario se efectuó la Campaña de Detección y Vacunación de Hepatitis Virales. Este trabajo tiene como objetivo caracterizar los principales factores de riesgo sexual (FRS) y parenteral (FRP) de los voluntarios asistentes a dicha campaña. Se llevó a cabo un estudio de corte transversal utilizando como instrumento un cuestionario validado. Se encontró que el 37,1%(IC 95%: 32,8-41,6%) de los encuestados refería al menos un FRS, siendo el más prevalente la presencia de relaciones sexuales inseguras con el 68,9% (61,6-75,6%). El 35,7% (31,4-40,1%) presentó al menos un FRP, destacándose aquí la utilización de piercings y tatuajes con el 74,6% (67,4-80,9%) y el 48% (40,3-55,7%) respectivamente. En los individuos mayores de 60 años,los hombres hicieron referencia a mayor exposición a FRS que las mujeres (37,2% vs 16,2%; p= 0,019); mientras que para los menores de 40 años las mujeres presentaron mayor exposición a FRP (64,5% vs 45,0%; p= 0,016). La prevalencia de factores de riesgo aumentó al disminuir la edad de los respondedores. Los jóvenes entre 18 y 30 años mostraron además, asociación entre los FRS y FRP (p= 0,01). Los factores de riesgo estudiados resultan diferenciarse según grupos etarios y género. Es necesario contemplar una educación dirigida a distintos grupos poblacionales, focalizando en las debilidades propias de cada uno(AU)

Viral liver disease is a problem and a challenge for physicians and their patients. A campaign for the detection and vaccination againstviral hepatitis was performed in August 2013 in the city of Rosario, Argentina. This work aims to characterize the main sexual (SRF)and parenteral (PRF) risk factors among those volunteers attending the campaign. A cross-sectional study was conducted using avalidated questionnaire. It was found that 37.1% (95% CI: 32.8-41.6%) of the respondents mentioned at least one SRF; the mostprevalent factor was the presence of unsafe sex with 68.9% (61.6-75.6%). On the other hand, 35.7% (31.4-40.1%) referred atleast one PRF, of which the most frequent were the use of piercings and tattoos with 74.6% (67.4-80.9%) and 48% (40,3-55.7%)respectively. Men reported higher exposure to SRF than women (37.2% vs 16.2%, p= 0.019) among individuals older than 60 years;while for those under 40 years, women were more likely to show PRF (64.5% vs 45.0%, p= 0.016). The prevalence of risk factorswas increased while decreasing age of respondents. Young people between 18 and 30 also showed association between SRF and PRF(p = 0,01). Risk factors studied varied according to age groups and genders. It is necessary to provide education targeted to differentpopulation groups, focusing on their particular characteristics and weaknesses of each of them(AU)

Expression of the FUT2 gene and CD44 marker in patients with oral lesions

The aim of this work was to investigate the FUT 2 gene, the secretor status and the expression of CD44 protein in epithelial cells obtain from saliva samples from patients with oral lesions (benign, pre-cancerous and cancerous lesions, n = 94). We analyzed polymorphisms of the FUT2 gene by allele specific oligonucleotide¨Cpolymerase chain reaction. The FUT2 gene encodes the ¦Á(1,2) fucosyltransferase (Se enzyme) that regulates the expression of ABH antigens in secretions. Generally speaking, being a non-secretor has several disadvantages with regard to metabolism and immune function. In this study, we found that oral pre-cancerous and cancerous lesions were increased among individuals with non-secretor status and nonsense mutation 428G¡úA. Fifty-one percent of the patients with oral pre-malignant and malignant lesions were non-secretors, in contrast with the healthy population (OR = 3.44). We observed a marginal association between secretor status and these lesions. Our study suggests that the lack of wild type FUT2 gene and a non-secretor status appear to be an associated risk marker for the development of oral cancer in patients with oral lesions (AU)

El objetivo del presente trabajo fue investigar la expresión del gen FUT 2 y del marcador

Ascaris lumbricoides: alteration of the erythrocyte superficial charge using the Partition Method in aqueous two-phase system.

Sialic acid is responsible for the negative charge of the erythrocyte. The decrease of sialic acid has hemodynamical and hemorheological importance. The aim was to study the effect of A. lumbricoides on the erythrocyte superficial charge using the Partition Method in aqueous two-phase system in order to indirectly evaluate the alteration of sialic acid in the red cells. We worked with five parasite extracts (AE) and larvae concentrate (LC). Erythrocyte superficial charge was studied by working with non-treated (Controls) and treated erythrocytes. The treatment consisted of incubating the erythrocytes with AE or LC for 30 minutes at 4 degrees C, 20 degrees C and 37 degrees C. The red cells were separated in a sensitive charge two-phase system (Dx/ PEG). The partition coefficient (P) of treated and untreated erythrocytes were calculated. The results showed a P decrease at the three temperatures for red cells treated with four of the AE. The remaining extract did change P values at any of the temperatures studied. The erythrocytes treated with LC showed a decrease in the P value at 37 degrees C and 4 degrees C but no change was observed at 25 degrees C. Statistical analysis concluded that P values were significantly lower in treated erythrocytes than in their corresponding untreated ones (p < 0.05). The Partition Method showed that this parasite alters the erythrocyte superficial charge which may indicate that it can catch sialic acid.

Ascaris lumbricoides: alteration of the erythrocyte superficial charge using the Partition Method in aqueous two-phase system / Ascaris lumbricoides: alteración de la carga superficial eritrocitaria usando el Método de Partición en sistemas bifásicos acuosos

Sialic acid is responsible for the negative charge of the erythrocyte. The decrease of sialic acid has hemodynamical and hemorheological importance. The aim was to study the effect of A. lumbricoides on the erythrocyte superficial charge using the Partition Method in aqueous two-phase system in order to indirectly evaluate the alteration of sialic acid in the red cells. We worked with five parasite extracts (AE) and larvae concentrate (LC). Erythrocyte superficial charge was studied by working with non-treated (Controls) and treated erythrocytes. The treatment consisted of incubating the erythrocytes with AE or LC for 30 minutes at 4 ºC, 20 ºC and 37 ºC. The red cells were separated in a sensitive charge two-phase system (Dx/ PEG). The partition coefficient (P) of treated and untreated erythrocytes were calculated. The results showed a P decrease at the three temperatures for red cells treated with four of the AE. The remaining extract did change P values at any of the temperatures studied. The erythrocytes treated with LC showed a decrease in the P value at 37 ºC and 4 ºC but no change was observed at 25 ºC. Statistical analysis concluded that P values were significantly lower in treated erythrocytes than in their corresponding untreated ones (p < 0.05). The Partition Method showed that this parasite alters the erythrocyte superficial charge which may indicate that it can catch sialic acid.

La disminución de ácido sialico, responsable de la carga negativa del eritrocito, tiene importancia hemodinámica y hemorreológica. El objetivo fue estudiar el efecto de A. lumbricoides sobre la carga superficial eritrocitaria aplicando el método de partición en sistemas bifásicos acuosos, a los fines de evaluar de manera indirecta la alteración de acido sialico de los eritrocitos. Se trabajó con 5 extractos del parásito adulto (EA) y con un concentrado de larvas (500-600 larvas/mL) (CL). Se estudió la carga superficial eritrocitaria, trabajando con eritrocitos no tratados y tratados. El tratamiento consistió en la incubación de los eritrocitos con EA o CL durante 30 minutos a 4 ºC, 25 ºC y 37 ºC. Los eritrocitos fueron sometidos a la separación en un sistema bifásico carga sensible constituido por Dx / PEG. Se calculó el coeficiente de reparto (P), de los eritrocitos sin tratar y tratados. Los resultados mostraron disminución de P a las 3 temperaturas, en hematíes tratados con 4 de los EA. El EA restante no modificó los valores de P a ninguna de las temperaturas estudiadas. CL produjo la disminución de P a 37 ºC y 4 ºC, pero no se observó modificación a 25 ºC. Los análisis estadísticos concluyeron que los valores de P son significativamente menores en los eritrocitos tratados que en los respectivos eritrocitos sin tratar (p < 0.05). El método de partición demostró que A. lumbricoides altera la carga superficial eritrocitaria lo que indicaría que el parásito, tanto en su estado adulto como en sus fases larvales, puede captar acido sialico.

[Serum sialic acid, cellular anionic charge and erythrocyte aggregation in diabetic and hypertensive patients]. / Acido siálico sérico, carga aniónica y agregación eritrocitaria en pacientes diabéticos e hipertensos.

The aim of this work was to study the relationship between serum sialic acid (SSA) and erythrocyte anionic charge (EAC) with erythrocyte aggregation in two groups: diabetic (DBT, n=20) and hypertensive (HT, n=21) patients, compared to a control group (n=20). We worked with anticoagulated blood with EDTA and serum. The erythrocyte aggregation was studied by microscopically observing and quantifying aggregates using an ASP (Aggregate Shape Parameter). The EAC was determined by binding an Alcian blue dye to the membrane sialic acid and SSA was determined by spectrophotometric method with an Erlich reactant. The values of ASP and SSA increased significantly in HT and DBT patients compared to the control group. The HT and DBT groups showed amorphous aggregates, evident in an alteration in the values of ASP, which were significantly higher ( p < 0.005) than in healthy patients. The EAC values were much lower in HT and DBT patients than in the control group (p < 0.0001). In this work, abnormalities in the erythrocyte aggregation could be detected by the values of ASP, EAC and SSA, which might be involved in vascular disorders of diseases such as hypertension and diabetes.

Ácido siálico sérico, carga aniónica y agregación eritrocitaria en pacientes diabéticos e hipertensos / Serum sialic acid, cellular anionic charge and erythrocyte aggregation in diabetic and hypertensive patients

El objetivo de este trabajo fue estudiar la relación del ácido siálico sérico (AS) y la carga aniónica eritrocitaria (CAE) con la agregación eritrocitaria en dos grupos de pacientes: diabéticos (DBT n= 20) e hipertensos (HTA n= 21), comparados con un grupo control (n= 20). Se trabajó con sangre anticoagulada con EDTA y suero. La agregación eritrocitaria se estudió por observación microscópica de los agregados y cuantificación a través de un parámetro de forma denominado ASP (Aggregation Shape Parameter). La CAE se determinó por unión a colorante alcian blue y el AS por método espectrofotométrico con reactivo de Erlich. Los valores de ASP y AS resultaron significativamente aumentados en los HTA y DBT respecto de los normales. Los HTA y DBT presentaron agregados amorfos, lo que se refleja en los valores alterados de ASP, significativamente mayores (p < 0.005) respecto de los individuos normales. Los valores de CAE resultaron significativamente inferiores en los HTA y DBT respecto del grupo control (p < 0.0001). En este trabajo se demostraron anormalidades en la agregación eritrocitaria, detectadas por los valores de ASP, CAE y AS que podrían estar involucradas en las complicaciones vasculares de vasculopatías como la hipertensión y la diabetes.

The aim of this work was to study the relationship between serum sialic acid (SSA) and erythrocyte anionic charge (EAC) with erythrocyte aggregation in two groups: diabetic (DBT, n=20) and hypertensive (HT, n=21) patients, compared to a control group (n=20). We worked with anticoagulated blood with EDTA and serum. The erythrocyte aggregation was studied by microscopically observing and quantifying aggregates using an ASP (Aggregate Shape Parameter). The EAC was determined by binding an Alcian blue dye to the membrane sialic acid and SSA was determined by spectrophotometric method with an Erlich reactant. The values of ASP and SSA increased significantly in HT and DBT patients compared to the control group. The HT and DBT groups showed amorphous aggregates, evident in an alteration in the values of ASP, which were significantly higher ( p< 0.005) than in healthy patients. The EAC values were much lower in HT and DBT patients than in the control group (p < 0.0001). In this work, abnormalities in the erythrocyte aggregation could be detected by the values of ASP, EAC and SSA, which might be involved in vascular disorders of diseases such as hypertension and diabetes.